DNA

Part:BBa_K5096070:Design

Designed by: Seonwoo Im   Group: iGEM24_Lambert-GA   (2024-09-30)


sg70


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the CRISPR sgRNA design tool from Benchling to generate the guide sequence, along with the sgRNA-tracr and sgRNA repeat from Marshall et al. (2018).



Source

M.tb

References